Review



amplex red cholesterol fluorometric assay kit  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 99
      Buy from Supplier

    Structured Review

    Thermo Fisher amplex red cholesterol fluorometric assay kit
    HBV-miR-3 expression leads to hepatocyte <t>cholesterol</t> build-up. A: Experimental design of the <t>fluorometric</t> cholesterol estimation assay performed for evaluating the expected increase in intracellular cholesterol post HBV-miR-3 expression in hepatocytes. B: Cellular cholesterol levels in cells (Huh7, HepG2) 48h after transfection with pHBV-miR-3, pcDNA3.1+, HBV 1.3x and microRNA-Scramble control (miR-SC). The cholesterol levels were normalised to respective total protein content. Bar graphs represent mean (±SD) of three independent experiments. C: Left panel: Hepatic lipid droplet accumulation visualised by BODIPY 493/503 staining in cells (Huh7, HepG2) 48 h post transfection with pHBV-miR-3 and pcDNA3.1+. The cells were counterstained with nuclear stain DAPI. Scale bar: 20 μM. Right panel: Quantitative analysis of BODIPY 493/503 fluorescence intensity was quantified in each micrograph and expressed as the mean ± standard errors (n = 6) in arbitrary units (a.u). ∗ P ≤ 0.05.
    Amplex Red Cholesterol Fluorometric Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 8424 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplex red cholesterol fluorometric assay kit/product/Thermo Fisher
    Average 99 stars, based on 8424 article reviews
    amplex red cholesterol fluorometric assay kit - by Bioz Stars, 2026-03
    99/100 stars

    Images

    1) Product Images from "HBV-miR-3 induces hepatic cholesterol accumulation by targeting ABCA1 : Evidence for potential benefits of statin usage"

    Article Title: HBV-miR-3 induces hepatic cholesterol accumulation by targeting ABCA1 : Evidence for potential benefits of statin usage

    Journal: Journal of Lipid Research

    doi: 10.1016/j.jlr.2025.100866

    HBV-miR-3 expression leads to hepatocyte cholesterol build-up. A: Experimental design of the fluorometric cholesterol estimation assay performed for evaluating the expected increase in intracellular cholesterol post HBV-miR-3 expression in hepatocytes. B: Cellular cholesterol levels in cells (Huh7, HepG2) 48h after transfection with pHBV-miR-3, pcDNA3.1+, HBV 1.3x and microRNA-Scramble control (miR-SC). The cholesterol levels were normalised to respective total protein content. Bar graphs represent mean (±SD) of three independent experiments. C: Left panel: Hepatic lipid droplet accumulation visualised by BODIPY 493/503 staining in cells (Huh7, HepG2) 48 h post transfection with pHBV-miR-3 and pcDNA3.1+. The cells were counterstained with nuclear stain DAPI. Scale bar: 20 μM. Right panel: Quantitative analysis of BODIPY 493/503 fluorescence intensity was quantified in each micrograph and expressed as the mean ± standard errors (n = 6) in arbitrary units (a.u). ∗ P ≤ 0.05.
    Figure Legend Snippet: HBV-miR-3 expression leads to hepatocyte cholesterol build-up. A: Experimental design of the fluorometric cholesterol estimation assay performed for evaluating the expected increase in intracellular cholesterol post HBV-miR-3 expression in hepatocytes. B: Cellular cholesterol levels in cells (Huh7, HepG2) 48h after transfection with pHBV-miR-3, pcDNA3.1+, HBV 1.3x and microRNA-Scramble control (miR-SC). The cholesterol levels were normalised to respective total protein content. Bar graphs represent mean (±SD) of three independent experiments. C: Left panel: Hepatic lipid droplet accumulation visualised by BODIPY 493/503 staining in cells (Huh7, HepG2) 48 h post transfection with pHBV-miR-3 and pcDNA3.1+. The cells were counterstained with nuclear stain DAPI. Scale bar: 20 μM. Right panel: Quantitative analysis of BODIPY 493/503 fluorescence intensity was quantified in each micrograph and expressed as the mean ± standard errors (n = 6) in arbitrary units (a.u). ∗ P ≤ 0.05.

    Techniques Used: Expressing, Transfection, Control, Staining, Fluorescence



    Similar Products

    amplex red cholesterol fluorometric assay kit  (Thermo Fisher)
    99
    Thermo Fisher amplex red cholesterol fluorometric assay kit
    HBV-miR-3 expression leads to hepatocyte <t>cholesterol</t> build-up. A: Experimental design of the <t>fluorometric</t> cholesterol estimation assay performed for evaluating the expected increase in intracellular cholesterol post HBV-miR-3 expression in hepatocytes. B: Cellular cholesterol levels in cells (Huh7, HepG2) 48h after transfection with pHBV-miR-3, pcDNA3.1+, HBV 1.3x and microRNA-Scramble control (miR-SC). The cholesterol levels were normalised to respective total protein content. Bar graphs represent mean (±SD) of three independent experiments. C: Left panel: Hepatic lipid droplet accumulation visualised by BODIPY 493/503 staining in cells (Huh7, HepG2) 48 h post transfection with pHBV-miR-3 and pcDNA3.1+. The cells were counterstained with nuclear stain DAPI. Scale bar: 20 μM. Right panel: Quantitative analysis of BODIPY 493/503 fluorescence intensity was quantified in each micrograph and expressed as the mean ± standard errors (n = 6) in arbitrary units (a.u). ∗ P ≤ 0.05.
    Amplex Red Cholesterol Fluorometric Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplex red cholesterol fluorometric assay kit/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    amplex red cholesterol fluorometric assay kit - by Bioz Stars, 2026-03
    99/100 stars
    		  Buy from Supplier
    fluorometric amplex red cholesterol assay kit  (Thermo Fisher)
    97
    Thermo Fisher fluorometric amplex red cholesterol assay kit
    HBV-miR-3 expression leads to hepatocyte <t>cholesterol</t> build-up. A: Experimental design of the <t>fluorometric</t> cholesterol estimation assay performed for evaluating the expected increase in intracellular cholesterol post HBV-miR-3 expression in hepatocytes. B: Cellular cholesterol levels in cells (Huh7, HepG2) 48h after transfection with pHBV-miR-3, pcDNA3.1+, HBV 1.3x and microRNA-Scramble control (miR-SC). The cholesterol levels were normalised to respective total protein content. Bar graphs represent mean (±SD) of three independent experiments. C: Left panel: Hepatic lipid droplet accumulation visualised by BODIPY 493/503 staining in cells (Huh7, HepG2) 48 h post transfection with pHBV-miR-3 and pcDNA3.1+. The cells were counterstained with nuclear stain DAPI. Scale bar: 20 μM. Right panel: Quantitative analysis of BODIPY 493/503 fluorescence intensity was quantified in each micrograph and expressed as the mean ± standard errors (n = 6) in arbitrary units (a.u). ∗ P ≤ 0.05.
    Fluorometric Amplex Red Cholesterol Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorometric amplex red cholesterol assay kit/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    fluorometric amplex red cholesterol assay kit - by Bioz Stars, 2026-03
    97/100 stars
    		  Buy from Supplier

    Image Search Results


    HBV-miR-3 expression leads to hepatocyte cholesterol build-up. A: Experimental design of the fluorometric cholesterol estimation assay performed for evaluating the expected increase in intracellular cholesterol post HBV-miR-3 expression in hepatocytes. B: Cellular cholesterol levels in cells (Huh7, HepG2) 48h after transfection with pHBV-miR-3, pcDNA3.1+, HBV 1.3x and microRNA-Scramble control (miR-SC). The cholesterol levels were normalised to respective total protein content. Bar graphs represent mean (±SD) of three independent experiments. C: Left panel: Hepatic lipid droplet accumulation visualised by BODIPY 493/503 staining in cells (Huh7, HepG2) 48 h post transfection with pHBV-miR-3 and pcDNA3.1+. The cells were counterstained with nuclear stain DAPI. Scale bar: 20 μM. Right panel: Quantitative analysis of BODIPY 493/503 fluorescence intensity was quantified in each micrograph and expressed as the mean ± standard errors (n = 6) in arbitrary units (a.u). ∗ P ≤ 0.05.

    Journal: Journal of Lipid Research

    Article Title: HBV-miR-3 induces hepatic cholesterol accumulation by targeting ABCA1 : Evidence for potential benefits of statin usage

    doi: 10.1016/j.jlr.2025.100866

    Figure Lengend Snippet: HBV-miR-3 expression leads to hepatocyte cholesterol build-up. A: Experimental design of the fluorometric cholesterol estimation assay performed for evaluating the expected increase in intracellular cholesterol post HBV-miR-3 expression in hepatocytes. B: Cellular cholesterol levels in cells (Huh7, HepG2) 48h after transfection with pHBV-miR-3, pcDNA3.1+, HBV 1.3x and microRNA-Scramble control (miR-SC). The cholesterol levels were normalised to respective total protein content. Bar graphs represent mean (±SD) of three independent experiments. C: Left panel: Hepatic lipid droplet accumulation visualised by BODIPY 493/503 staining in cells (Huh7, HepG2) 48 h post transfection with pHBV-miR-3 and pcDNA3.1+. The cells were counterstained with nuclear stain DAPI. Scale bar: 20 μM. Right panel: Quantitative analysis of BODIPY 493/503 fluorescence intensity was quantified in each micrograph and expressed as the mean ± standard errors (n = 6) in arbitrary units (a.u). ∗ P ≤ 0.05.

    Article Snippet: The second aliquot was subjected to lipid extraction as described earlier ( ) followed by estimation of total cellular cholesterol content using the Amplex red cholesterol fluorometric assay kit (ThermoFisher Scientific).

    Techniques: Expressing, Transfection, Control, Staining, Fluorescence